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validation novel plant genotypes seed stocks authentication plants pgi proteintech group  (Proteintech)


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    Proteintech validation novel plant genotypes seed stocks authentication plants pgi proteintech group
    Validation Novel Plant Genotypes Seed Stocks Authentication Plants Pgi Proteintech Group, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17330+1+ap/Pepsinogen+I+Antibody/pmc12381190__41467_2025_62588_MOESM6_ESM-38-70-79
    Average 91 stars, based on 3 article reviews
    validation novel plant genotypes seed stocks authentication plants pgi proteintech group - by Bioz Stars, 2026-09
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    Article Title: Clinical significance of spasmolytic polypeptide-expressing metaplasia and intestinal metaplasia in Epstein-Barr virus-associated and Epstein-Barr virus-negative gastric cancer.
    Article Snippet: Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer Yu Zhang MD, Jian-ning Chen MD, Min Dong MD, Zhi-gang Zhang MD, Yi-wang Zhang MD, PhD, Jun-yan Wu MD, Hong Du MD, Hai-gang Li MD, Yan Huang MD, Chun-kui Shao MD, PhD PII: S0046-8177(17)30067-9 DOI: doi: 10.1016/j.humpath.2017.02.016 Reference: YHUPA 4147 To appear in: Human Pathology Received date: 14 November 2016 Revised date: 30 January 2017 Accepted date: 23 February 2017 Please cite this article as: Zhang Yu, Chen Jian-ning, Dong Min, Zhang Zhi-gang, Zhang Yi-wang, Wu Jun-yan, Du Hong, Li Hai-gang, Huang Yan, Shao Chun-kui, Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer, Human Pathology (2017), doi: 10.1016/j.humpath.2017.02.016 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival
    Article Snippet: Pepsinogen 5, group I (pepsinogen A) , Rabbit, P , 17330-1-AP; Proteintech Europe, Manchester, UK , 50 , , 4°C on , Chief cells.



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    validation novel plant genotypes seed stocks authentication plants pgi proteintech group  (Proteintech)
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    Proteintech validation novel plant genotypes seed stocks authentication plants pgi proteintech group
    Validation Novel Plant Genotypes Seed Stocks Authentication Plants Pgi Proteintech Group, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17330+1+ap/Pepsinogen+I+Antibody/pmc12381190__41467_2025_62588_MOESM6_ESM-38-70-79
    Average 91 stars, based on 1 article reviews
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    rabbit anti pepsinogen1  (Proteintech)
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    Figure 1. Bmi1-expressing cells were mainly located in the isthmus of the stomach and were unique from other differentiated or stem cells. (A) Immunohistochemistry for BMI1 in the normal human gastric epithelium. The location of BMI1-positive cells and the distribution ratio in the human gastric epithelium were plotted (n = 3). Scale bars: 50 μm. (B) Immunohistochemistry for GFP in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Bar graphs show the distribution of Bmi1+/YFP+ cells in the gastric epithelium (n = 3). Scale bars: 10 μm. (C) Immunofluorescent double staining for GFP and gastric differentiated cell markers: Muc5AC, chromogranin A (ChgA), Dclk1, Tff2, proton pump (PP) and <t>pepsinogen1</t> (Pga1) in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Scale bars: 10 μm. (D) Immunofluorescent double staining for Bmi1 and GFP on day 1 after 0.1 mg/g body weight tamoxifen injection in Lgr5-EGFP-CreERT mice and eR1-CreERT; Rosa26-YFP mice. Scale bars: 10 μm.
    Rabbit Anti Pepsinogen1, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti pepsinogen 5 pga5  (Proteintech)
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    (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells <t>(PGA5</t> (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.
    Rabbit Anti Pepsinogen 5 Pga5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pepsinogen 5  (Proteintech)
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    (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells <t>(PGA5</t> (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.
    Pepsinogen 5, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17330+1+ap/Pepsinogen+I+Antibody/pmc05597207-5-0-11
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    17330 1 ap  (Proteintech)
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    Proteintech 17330 1 ap
    (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells <t>(PGA5</t> (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.
    17330 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17330+1+ap/Pepsinogen+I+Antibody/pmc05597207-5-10-11
    Average 91 stars, based on 1 article reviews
    17330 1 ap - by Bioz Stars, 2026-09
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    rabbit anti pepsinogen  (Proteintech)
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    Proteintech rabbit anti pepsinogen
    (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells <t>(PGA5</t> (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.
    Rabbit Anti Pepsinogen, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17330+1+ap/Pepsinogen+I+Antibody/pm28300576-68-6-12
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    Figure 1. Bmi1-expressing cells were mainly located in the isthmus of the stomach and were unique from other differentiated or stem cells. (A) Immunohistochemistry for BMI1 in the normal human gastric epithelium. The location of BMI1-positive cells and the distribution ratio in the human gastric epithelium were plotted (n = 3). Scale bars: 50 μm. (B) Immunohistochemistry for GFP in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Bar graphs show the distribution of Bmi1+/YFP+ cells in the gastric epithelium (n = 3). Scale bars: 10 μm. (C) Immunofluorescent double staining for GFP and gastric differentiated cell markers: Muc5AC, chromogranin A (ChgA), Dclk1, Tff2, proton pump (PP) and pepsinogen1 (Pga1) in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Scale bars: 10 μm. (D) Immunofluorescent double staining for Bmi1 and GFP on day 1 after 0.1 mg/g body weight tamoxifen injection in Lgr5-EGFP-CreERT mice and eR1-CreERT; Rosa26-YFP mice. Scale bars: 10 μm.

    Journal: The Journal of pathology

    Article Title: Bmi1 marks gastric stem cells located in the isthmus in mice.

    doi: 10.1002/path.5244

    Figure Lengend Snippet: Figure 1. Bmi1-expressing cells were mainly located in the isthmus of the stomach and were unique from other differentiated or stem cells. (A) Immunohistochemistry for BMI1 in the normal human gastric epithelium. The location of BMI1-positive cells and the distribution ratio in the human gastric epithelium were plotted (n = 3). Scale bars: 50 μm. (B) Immunohistochemistry for GFP in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Bar graphs show the distribution of Bmi1+/YFP+ cells in the gastric epithelium (n = 3). Scale bars: 10 μm. (C) Immunofluorescent double staining for GFP and gastric differentiated cell markers: Muc5AC, chromogranin A (ChgA), Dclk1, Tff2, proton pump (PP) and pepsinogen1 (Pga1) in 8-week-old Bmi1-CreERT; Rosa26-YFP mice 1 day after 0.1 mg/g body weight tamoxifen injection. Scale bars: 10 μm. (D) Immunofluorescent double staining for Bmi1 and GFP on day 1 after 0.1 mg/g body weight tamoxifen injection in Lgr5-EGFP-CreERT mice and eR1-CreERT; Rosa26-YFP mice. Scale bars: 10 μm.

    Article Snippet: Primary antibodies were obtained from the indicated suppliers: goat anti-GFP (×500; ab6673, Abcam, Cambridge, MA, USA), rat anti-Ki67 (×100; M7249, Dako), anti-Muc5AC (×100; ab3649, Abcam), rabbit anti-chromogranin A (×100; ab15160, Abcam), rabbit anti-Dclk1 (×200; ab31704, Abcam), mouse anti-spasmolytic polypeptide IgM (×100; ab49536, Abcam), mouse anti-proton pump (×1; D031-3H, Medical & Biological Laboratories, Nagoya, Aichi, Japan), rabbit anti-pepsinogen1 (×100; 17330-1-AP, Proteintech, Rosemont, IL, USA), sheep anti-pepsinogen2 (×10000; ab9013, Abcam), rabbit anti-human Bmi1 (×400; #6964S, Cell Signaling Technology, Danvers, MA, USA), goat anti-murine Bmi1 (×300; ab115251, Abcam) and rabbit anti-cleaved caspase3 (×100; #9664, Cell Signaling Technology).

    Techniques: Expressing, Immunohistochemistry, Injection, Double Staining

    (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells (PGA5 (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.

    Journal: PLoS ONE

    Article Title: The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival

    doi: 10.1371/journal.pone.0184514

    Figure Lengend Snippet: (A) IHC and ISH showing similar expression patterns for clusterin (brown) protein and mRNA, examined in control (n = 8) and hypergastrinemic PPI-rats (n = 6). (B) Number of CLU-positive/VMAT2(neuroendocrine (NE))-positive cells and CLU-positive/VMAT2(NE)-negative cells per oxyntic gland (n = 4 rats per group). (C) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing scarce CLU (green) expression in ECL cells (VMAT2-positive (red)). (D) Double immunofluorescence staining of oxyntic mucosa from hypergastrinemic PPI-rats showing CLU (green) expression in groups of chief cells (MIST1-positive (purple)) and triple immunofluorescence staining showing CLU (green) expression in proliferating (PCNA-positive (light blue)) chief cells (PGA5 (red)). Ctr = control; PPI = PPI-induced hypergastrinemia; CCK2Ra = CCK2R antagonist; PPI+CCK2Ra = PPI-induced hypergastrinemia+CCK2R antagonist. Data presented as means ± SEM. *, ** and # = ANOVA with Bonferroni-adjusted p value < 0.05. (*comparison of CLU+/NE+ cells per gland in control vs other groups individually; **comparison of CLU+/NE- cells per gland in PPI vs control or CCK2Ra; #comparison of CLU+/NE- cells per gland in PPI+CCK2Ra vs CCK2Ra.) Nuclei were counterstained with hematoxylin (blue) or DAPI (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = (A) 100 μm; (C, D) 50 μm.

    Article Snippet: The primary antibodies rabbit anti-CLU (H-330, sc-8354, Santa Cruz Biotechnology Inc., Dallas, TX) and rabbit anti-pepsinogen 5 (PGA5) (17330-1-AP, Proteintech Europe, Manchester, United Kingdom) were detected with tyramide signal amplification TM PLUS Fluorescence Kit (NEL760001KT, Perkin Elmer, Waltham, MA) according to manufacturer’s instructions.

    Techniques: Expressing, Control, Double Immunofluorescence Staining, Immunofluorescence, Staining, Comparison

    (A, B, C) HDC protein level (A) and CLU protein level (B) in whole mucosa lysates by targeted mass spectrometry and plasma (C) by ELISA from control (Ctr) (n = 9), PPI-induced hypergastrinemic (PPI) (n = 6), CCK2R-antagonized (CCK2Ra) (n = 5–8) and CCK2R-antagonized PPI-induced hypergastrinemic (PPI+CCK2Ra) (n = 6–8) rats. Each dot represents an individual animal and the black lines mark median. (D) ISH (n = 4) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized rats showing expression of clusterin (brown). (E) ISH (n = 5) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized hypergastrinemic PPI-rats showing expression of clusterin (brown) (compare (D) and (E) to control and hypergastrinemic PPI-rats in ). (F-H) Number per oxyntic gland of (F) CLU-positive cells in IHC-stained sections (n = 5–8 rats per group), (G) dual CLU-positive/PGA5-positive cells (n = 4 rats per group), and (H) dual CLU-positive/PCNA-positive cells (n = 4 rats per group). Data presented as means ± SEM. *ANOVA with Tukey-adjusted p value < 0.05. Nuclei were counterstained with hematoxylin (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = 100 μm.

    Journal: PLoS ONE

    Article Title: The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival

    doi: 10.1371/journal.pone.0184514

    Figure Lengend Snippet: (A, B, C) HDC protein level (A) and CLU protein level (B) in whole mucosa lysates by targeted mass spectrometry and plasma (C) by ELISA from control (Ctr) (n = 9), PPI-induced hypergastrinemic (PPI) (n = 6), CCK2R-antagonized (CCK2Ra) (n = 5–8) and CCK2R-antagonized PPI-induced hypergastrinemic (PPI+CCK2Ra) (n = 6–8) rats. Each dot represents an individual animal and the black lines mark median. (D) ISH (n = 4) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized rats showing expression of clusterin (brown). (E) ISH (n = 5) and IHC (n = 8) of oxyntic mucosa from CCK2R-antagonized hypergastrinemic PPI-rats showing expression of clusterin (brown) (compare (D) and (E) to control and hypergastrinemic PPI-rats in ). (F-H) Number per oxyntic gland of (F) CLU-positive cells in IHC-stained sections (n = 5–8 rats per group), (G) dual CLU-positive/PGA5-positive cells (n = 4 rats per group), and (H) dual CLU-positive/PCNA-positive cells (n = 4 rats per group). Data presented as means ± SEM. *ANOVA with Tukey-adjusted p value < 0.05. Nuclei were counterstained with hematoxylin (blue). The basal zone (~100 μm from the gland bottom) is highlighted with a dotted line. Scale bars = 100 μm.

    Article Snippet: The primary antibodies rabbit anti-CLU (H-330, sc-8354, Santa Cruz Biotechnology Inc., Dallas, TX) and rabbit anti-pepsinogen 5 (PGA5) (17330-1-AP, Proteintech Europe, Manchester, United Kingdom) were detected with tyramide signal amplification TM PLUS Fluorescence Kit (NEL760001KT, Perkin Elmer, Waltham, MA) according to manufacturer’s instructions.

    Techniques: Mass Spectrometry, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Expressing, Staining